Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after <sup>60</sup>Co γ-ray radiation at 12 Gy

YAN Yue; YE Yifan; WANG Lei; JIANG Huiqian; ZHUANG Xinli; CHEN Xiaoying; HE Changhao; ZHANG Shujing; XU Wenhui; HU Sumin

doi:10.3969/j.issn.2095-6606.2022.06.010

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Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after ⁶⁰Co γ-ray radiation at 12 Gy

更新时间：2022-12-03

- Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after ⁶⁰Co γ-ray radiation at 12 Gy
- Modern Chinese Clinical Medicine Vol. 29, Issue 6, Pages: 44-49(2022)
- 作者机构：
  
  1.北京中医药大学中医学院　北京　100029
  2.北京中医药大学北京中医药研究院
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.2095-6606.2022.06.010
  CLC： R2-03
- Published：30 November 2022，
  
  Received：26 February 2022，
- 稿件说明：
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YAN Yue, YE Yifan, WANG Lei, et al. Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after ⁶⁰Co γ-ray radiation at 12 Gy. [J]. Modern Chinese Clinical Medicine 29(6):44-49(2022)
DOI：

YAN Yue, YE Yifan, WANG Lei, et al. Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after ⁶⁰Co γ-ray radiation at 12 Gy. [J]. Modern Chinese Clinical Medicine 29(6):44-49(2022) DOI： 10.3969/j.issn.2095-6606.2022.06.010.

摘要

目的

研究

Co γ射线对睾丸支持细胞(Sertoli细胞)炎性因子分泌水平的影响，并探究益气解毒方治疗后Sertoli细胞分泌功能损伤的防护机制。

方法

Sertoli细胞分为正常组(NC组)、模型组(IR组)、益气解毒高剂量组(YQJD-H组)、益气解毒中剂量组(YQJD组)、益气解毒低剂量组(YQJD-L组)、核因子激活的B细胞的k－轻链增强抑制剂组(Bay组)、中药＋Bay组(Y＋B组)。各组以相应条件培养24 h后，除NC组外，使用12 Gy

Co γ射线进行一次性照射，建立Sertoli细胞辐射损伤模型。CCK8法检测细胞活力，Elisa法检测细胞上清液中白细胞介素－1β(Interleukin－1β，IL－1β)、白细胞介素－6(Interleukin－6

IL－6)、肿瘤坏死因子－α(Tumor Necrosis Factor－α，TNF－α)、转化生长因子－β(Transforming Growth Factor－β，TGF－β)的含量，WB法检测NF－κB/IκB α通路蛋白表达水平。

结果

照射后24 h，与NC组比较，IR组Sertoli细胞增殖能力下降(

0.01)，IL－1β、IL－6、TNF－α、TGF－β的分泌量均明显升高(

0.01)；NF－κB、磷酸化NF－κB蛋白表达水平均明显升高(

0.01)，IκBα蛋白表达水平明显降低(

0.01)；与IR组比较，益气解毒方能够升高照射后Sertoli细胞的存活率(

0.01)，YQJD组、Bay组、Y＋B组细胞分泌的IL－1β、IL－6、TNF－α、TGF－β的量均明显降低(

0.05)；YQJD组、Bay组、Y＋B组细胞的NF－κB蛋白表达水平明显降低(

0.05，

0.01，

0.01)，YQJD组、Y＋B组细胞的磷酸化NF－κB蛋白表达水平均明显降低(

0.01)，YQJD组、Bay组、Y＋B组细胞的IκBα蛋白表达水平均明显升高(

0.01)。

结论

12 Gy

Co γ射线降低Sertoli细胞增殖能力，使其分泌功能紊乱，而益气解毒方能通过调节NF－κB/IκB α通路，减轻Sertoli细胞受到的辐射损伤，在一定程度上促进睾丸炎性微环境恢复稳定。

Abstract

Objective

To study the effect of

Co γ-ray on secretion of inflammatory factors by Sertoli cells

and to explore the protective mechanism of Yiqi Jiedu Decoction (Qi-Boosting Toxin-Resolving Decoction) on the secretory function of Sertoli cells injured by the radiation.

Methods

Sertoli cells were divided into Normal Control (NC) group

Ionizing Radiation (IR) group

Yiqi Jiedu Decoction high dosage (YQJD-H) group

Yiqi Jiedu Decoction middle dosage (YQJD) group

Yiqi Jiedu Decoction low dosage (YQJD-L) group

NF-κB inhibitor (Bay) group and Yiqi Jiedu + NF-κB inhibitor (Y+ B) group. The groups were cultured under corresponding conditions for 24 hours. Then

radiation-damaged cell models were created by putting all groups except the NC group under

Co γ-ray radiation at 12 Gy. The CCK8 method was used to detect the cell viability of the Sertoli cells

Elisa to detect the content of IL-1β

IL-6

TNF-α

TGF-β in cell supernatant

and Western Blot to detect the protein expression level of the NF-κB/IκB α pathway.

Results

24 hours after irradiation

compared with the NC group

the proliferation of Sertoli cells in the IR group decreased (

0.01). The secretion of IL-1β

IL-6

TNF-α

TGF-β increased (

0.01). The protein expression level of NF-κB and phosphorylated NF-κB increased (

0.01). The protein expression level of IκB α decreased (

0.01). A comparison with the IR group reveals that Yiqi Jiedu decoction can increase the cell viability of Sertoli cells (

0.01). It can also significantly reduce the content of IL-1β

IL-6

TNF-α

TGF-β (

0.01

0.05

0.01). The protein expression level of NF-κBin in the YQJD group

Bay group and Y+ B group decreased (

0.05

0.001

0.01). The protein expression level of phosphorylated NF-κB in the YQJD group and Y+ B group decreased (

0.01). The protein expression level of IκB α in the YQJD group

Bay group and Y+ B group increased.

Conclusion

Co γ-ray radiation at 12 Gy reduced the proliferation ability of Sertoli cells and disordered their secretory function. Yiqi Jiedu Decoction can significantly reduce the radiation damage to Sertoli cells by regulating NF-κB/IκB α pathway

and promote the restoration and stability of testicular microenvironment to a certain extent.

关键词

益气解毒方电离辐射Sertoli细胞炎性因子NF－κB/IκB α通路

Keywords

Yiqi Jiedu DecoctionirradiationSertoli cellsinflammatory factorNF-κB/IκB α pathway

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Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after 60Co γ-ray radiation at 12 Gy

DOI：10.3969/j.issn.2095-6606.2022.06.010

摘要

Abstract

关键词

Keywords

references

Protective effect and mechanism of Qi-Boosting Toxin-Resolving Decoction on secretory function of Sertoli cells after ⁶⁰Co γ-ray radiation at 12 Gy