抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响

廖昭铭; 江锋; 叶永安; 靳耀英; 李忻

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抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响

中医临床基础研究 | 更新时间：2021-05-20

- 抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响
- Influence of Kangxianyi’ai Formula on metabolism of extracellular matrix in rats with liver fibrosis
- 现代中医临床 2009年16卷第2期页码：9-12
- 作者机构：
  
  1. 北京中医药大学
  2. 北京中医药大学东直门医院消化科
  3. 中日友好医院临床医学研究所
- 作者简介：
- 基金信息：
  
  高等学校博士学科点专项科研基金资助课题（No.20060026010）()
- DOI：
  中图分类号： R285.5
- 纸质出版日期：2009-02-01，
- 稿件说明：
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廖昭铭, 江锋, 叶永安, 等. 抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响[J]. 现代中医临床, 2009,16(2):9-12.

LIAO Zhao-ming~1, JIANG Feng~2, YE Yong-an~2, et al. Influence of Kangxianyi’ai Formula on metabolism of extracellular matrix in rats with liver fibrosis[J]. 2009,16(2):9-12.
廖昭铭, 江锋, 叶永安, 等. 抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响[J]. 现代中医临床, 2009,16(2):9-12. DOI：

LIAO Zhao-ming~1, JIANG Feng~2, YE Yong-an~2, et al. Influence of Kangxianyi’ai Formula on metabolism of extracellular matrix in rats with liver fibrosis[J]. 2009,16(2):9-12. DOI：

摘要

目的研究抗纤抑癌方对肝纤维化大鼠细胞外基质代谢的影响

探讨其抗肝纤维化的作用机制。方法以二乙基亚硝胺诱发大鼠肝纤维化模型

设复方鳖甲软肝片和秋水仙碱为对照药

观察抗纤抑癌方对该模型大鼠肝脏组织病理的影响

并通过免疫组化方法测定各组大鼠肝脏Ⅰ型胶原(CollagenⅠ)、Ⅳ型胶原(CollagenⅣ)、基质金属蛋白酶-1(MMP-1)和金属蛋白酶组织抑制因子-1(TIMP-1)的表达。结果抗纤抑癌方组MMP-1表达明显高于模型对照组和鳖甲软肝片组

CollagenⅠ、TIMP-1表达明显低于模型对照组

CollagenⅣ表达明显低于模型对照组和鳖甲软肝片组。结论抗纤抑癌方具有明显的抗肝纤维化作用

其作用机制可能与增加MMP-1表达

降低TIMP-1表达

促进细胞外基质降解

减少胶原沉积等有关。

Abstract

Objective To study the influence of Kangxianyi’ai Formula on metabolism of extracellular matrix in rats with liver fibrosis

and discuss the mechanism of its effect of anti-fibrosis.Methods The rat model of liver fibrosis was established by using diethylnitrosamine.Fufangbeijiaruangan Tablets and colchicine were taken as the control drugs.The influence of Kangxianyi’ai Formula on the histopathological changes of the liver in model rats were observed and the expressions of collagenⅠ

collagenⅣ

MMP-1 and TIMP-1 in rats of different groups were detected by using immunohistochemistry assay.Results In Kangxianyi’ai Formula group the expression of MMP-1 was significantly higher than that in the model control group or Fufangbeijiaruangan Tablets group respectively

the expressions of collagenⅠand TIMP-1 were significantly lower than those in the model control group

and the expression of collagenⅣwas significantly lower than that in the model control group or Fufangbeijiaruangan Tablets group. Conclusion Kangxianyi’ai Formula has significant effect of anti-fibrosis

and the mechanism may be related to that it can increase the expression of MMP-1

decrease the expression of TIMP-1

promote the degradation of extracellular matrix and reduce the aggradation of collagen.

关键词

肝纤维化细胞外基质抗纤抑癌方大鼠

Keywords

liver fibrosisextracellular matrixKangxianyi’ai Formularats

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